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P-95: Flow Cytometry Analysis of Bovine Semen:A Qualitative Study
Background: Although AI practices have been introduced little over 60 years, the success rate remains relatively low. This might be due to the exclusive selection of semen based on motility analysis. Recent advancement in sperm sexing using flow cytometry with an increased throughput from next generation cell sorters, made use of this technology in studding sperm qualitative aspects other than ...
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T cells are an essential cellular component of the immune system. When T cells encounter antigen and receive co-stimulatory signals, they become activated, proliferate and produce cytokines. Flow cytometry is a valuable research tool in analyzing T cell functions including proliferation, survival and cytotoxicity as well as cytokine production and cell signaling. This article has reviewed the u...
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Described are four widely used procedures to analyze the cell cycle by flow cytometry. The first two are based on univariate analysis of cellular DNA content following cell staining with either propidium iodide (PI) or 4',6'-diamidino-2-phenylindole (DAPI) and deconvolution of the cellular DNA content frequency histograms. This approach reveals distribution of cells in three major phases of the...
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Perturbation of the tubulin/microtubule dynamic in cells is perhaps the single most important mode of action of anticancer drugs. Standard methods for identifying and evaluating compounds for their ability to alter tubulin polymerization are low throughput, labor intensive, expensive, or make their assessment in vitro. Here we report a method to rapidly quantify the extent of tubulin polymeriza...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1979
ISSN: 0022-1554,1551-5044
DOI: 10.1177/27.1.374589